Development of morphological marker on natural interspecific hybrid seedling of Acacia (Acacia mangium × Acacia auriculiformis) verified by molecular marker

Abstract Development of the hybridization between two different species Acacia mangium and auriculiformis in Indonesia started 2009 by establishing an open-pollinated orchard to produce natural hybrid seed Wonogiri District, Central Java Province. The design was alternate row A. with a spacing 5 × meters. produced putative seeds firstly 2013. Based on flowering synchronization species, from 38 sample trees were taken identify their character seedling morphology obtain hybrid. morphological characteristics observed leaf development patterns emergence true ( pinnate ) until pseudo phyllode emerged. then verified using Simple Sequence Repeat (SSR) marker Characterized Amplified Region (SCAR) auriculiformis. result showed that presence 4- pattern as 100% pure opposite In comparison, + 40% final estimation production at first about 3.1% approximately based pattern.